Parameter Estimation in Different Enzyme Reactions
نویسندگان
چکیده
Enzyme kinetic parameters have been estimated using MATLAB software via the Wilkinson nonlinear regression technique. The MATLAB script file written to implement this technique is short and very straightforward. Several software tools are commercially available for this purpose, with many graphical user interface (GUI) features. A routine use of these packages might offer immediate satisfaction of interactive hands-on experience; but in some cases the researcher might wish to write his/her own code and compare the results for further confirmation. Today MATLAB is in use in almost all the schools and laboratories as a standard software tool. So this paper is aimed at helping enzyme researchers to make use of this powerful software for estimation of parameters. It enables the incorporation of the analytical steps behind parameter estimation in an easy-to-follow manner and furnishes better visualization.
منابع مشابه
Bayesian Estimation of Shift Point in Shape Parameter of Inverse Gaussian Distribution Under Different Loss Functions
In this paper, a Bayesian approach is proposed for shift point detection in an inverse Gaussian distribution. In this study, the mean parameter of inverse Gaussian distribution is assumed to be constant and shift points in shape parameter is considered. First the posterior distribution of shape parameter is obtained. Then the Bayes estimators are derived under a class of priors and using variou...
متن کاملIntegration of Enzyme Kinetic Data from Various Sources
We describe a workflow to translate a given metabolic network into a kinetic model; the model summarises kinetic information collected from different data sources. All reactions are modelled by convenience kinetics; where detailed kinetic laws are known, they can also be incorporated. Confidence intervals and correlations of the resulting model parameters are obtained from Bayesian parameter es...
متن کاملTarget Tracking with Unknown Maneuvers Using Adaptive Parameter Estimation in Wireless Sensor Networks
Abstract- Tracking a target which is sensed by a collection of randomly deployed, limited-capacity, and short-ranged sensors is a tricky problem and, yet applicable to the empirical world. In this paper, this challenge has been addressed a by introducing a nested algorithm to track a maneuvering target entering the sensor field. In the proposed nested algorithm, different modules are to fulfill...
متن کاملPiecewise affine approximations of fluxes and enzyme kinetics from in vivo
Owing to the ever increasing amount of available information on metabolic networks, and in particular to the increase in information content from in vivo C dynamic labeling experiments, this work investigates the problem of reconstructing dynamic fluxes and enzyme kinetics. The model structure is based on the use of piecewise affine approximations. The optimization procedure at the basis of the...
متن کاملOnline State Space Model Parameter Estimation in Synchronous Machines
The purpose of this paper is to present a new approach based on the Least Squares Error method for estimating the unknown parameters of the nonlinear 3rd order synchronous generator model. The proposed method uses the mathematical relationships between the machine parameters and on-line input/output measurements to estimate the parameters of the nonlinear state space model. The field voltage is...
متن کامل